In vivo studies of the angiogenic potential of Mandur Bhasma

https://doi.org/10.53730/ijhs.v6nS2.4971

Authors

  • Ekta Tomar Associate Professor, Dept. Of Rasashastra & Bhaishjya Kalpana, D Y Patil University, School Of Ayurveda, Nerul, Navi Mumbai, India
  • Sonali Wairagade Professor & HOD, Department of Kayachikitsa, Datta Meghe Ayurved Medical College Hospital and Research Centre, Wanadongri, Nagpur (MS), India
  • Arachana Gharote Professor & HOD, Dept. Of Rasashastra & Bhaishjya Kalpana, D Y Patil University, School Of Ayurveda, Nerul, Navi Mumbai, India
  • Ranjit S. Ambad Department of Biochemistry, Datta Meghe Medical College, Shalinitai Meghe Hospital & Research Centre Wanadongri, Hingana, Nagpur-441110, Maharashtra, India
  • Dhruba Hari Chandi Assistant Professor Dept. of Microbiology Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences Sawangi (Meghe), Wardha, India

Keywords:

angiogenic potential, CAM model, chick embryo, Mandur Bhasma

Abstract

Background: Mandur Bhasma is a herbo-mineral compound. It is prepared by Putapaka method. It is described as Raktasanjanan. In the current study, Mandur Bhasma was prepared with a standardized method w.s.r to Rasatarangini and an experimental study was done to observe the Angiogenic property of Mandur Bhasma. The current study will analyze angiogenic potential of Mandur Bhasma using chick CAM model. This research is intended to study the possible role of Mandur Bhasma on angiogenesis and establishing properties of Mandur Bhasma as an angiogenic by newer means. The experimental study inside the egg shell will be carried out on a membrane known as “chorioallantoic membrane”. Objectives:To Prepare Mandur Bhasma, Physicochemical and Analytical study of  Mandur Bhasma, To verify the angiogenic potential of Mandur bhasma using the chicken, horioallantoic membrane (CAM) model, To compare  Angeogenic potential of Mandur bhasma with standard drug progesterone. Methodology: Relevant classical literature regarding Mandur will be reviewed and the data will be collected. Mandur Shodhan with Gomutra and Mandur Maran with Triphala decoction will be done. Analytical Study like Organoleptic Test for Rasa, Gandha, Varna, Sparsha, Physicochemical Tests and other analytical test like ICP-AES /ICPMS, XRD structure of Bhasma, EDAX-NANO Particle Size will be done.

Downloads

Download data is not yet available.

References

Joshi, G. Y. (2003). Carak Samhita (6th ed., Vol. 1,2). Pune, Maharastra: Vaidyamitra prakashan.;Siddhisthan.

Ambikadattashastri, K. (2016). Sushruta samhita (Vol. 1,2). Varanasi: Chaukhmbha sanskrit sansthan.;Sutrasthan.

The Ayurvedic Pharmacopoeia of India (1st ed., Vol. VII). (2010). New Delhi: Govt. ofIndia, Ministry of Health and Family Welfare, Dept. of Ayurveda, Yoga & Naturopathy,Unani, Siddha and Homoeopathy (AYUSH).

Kashinathshastrina, P. (2000). Rasatarangini. Delhi: Motilal Banarasidas.

Dole, D. A. (2006). Rasa Ratna Samuccaya (1st ed.). Varanasi: Chaukhambha Anskrit series office

Tripathi, I. (n.d.). Rasarnava. Varanasi: Chaukhambha Saskrit Series Office.

Kashinathshastrina, P. (2000). Rasatarangini. Delhi: Motilal Banarasidas.

Chaube, D. (n.d.). Bruhat Rasa Raja Sundar (3rd ed., Ser. 2000). Varanasi: Chaukhambha Oriantalia.

Mookerji, B. (2001). Rasa-Jala-Nidhi (1st ed.). Delhi: Parimal Publication

Ribatti D. The Chick Embryo Chorioallantoic Membrane as an In Vivo Assay to Study Antiangiogenesis. Pharmaceuticals (Basel) 2010;3:482–513.

Ribatti D. The chick embryo chorioallantoic membrane as a model for tumor biology. Exp. Cell Res. 2014;328:314–324.

Liu M., Xie S., Zhou J. Use of animal models for the imaging and quantification of angiogenesis. Exp. Anim. 2018;67:1–6.

Ribatti D. The chick embryo chorioallantoic membrane (CAM). A multifaceted experimental model. Mech. Dev. 2016;141:70–77.

Cimpean A.M., Ribatti D, Raica M. The chick embryo chorioallantoic membrane as a model to study tumor metastasis. Angiogenesis. 2008;11:311–319.

Deryugina E.I., Quigley J.P. Chapter 2. Chick Embryo Chorioallantoic Membrane Models to Quantify Angiogenesis Induced by Inflammatory and Tumor Cells or Purified Effector Molecules. Methods Enzymol. 2008;444:21–41.

Published

23-03-2022

How to Cite

Tomar, E., Wairagade, S., Gharote, A., Ambad, R. S., & Chandi, D. H. (2022). In vivo studies of the angiogenic potential of Mandur Bhasma. International Journal of Health Sciences, 6(S2), 35–42. https://doi.org/10.53730/ijhs.v6nS2.4971

Issue

Section

Peer Review Articles