Quantification of DNA profiling from burnt remnants
Keywords:
burnt bodies, quantitative analysis, DNA profiling, burnt dead bodies, extraction method, RT-PCR, crime sceneAbstract
In crime scene investigation, various evidences are attained in distinct forms by forensic experts. These evidences are often recovered in contaminated form, decomposed or partial form especially skelton remnants. In homicidal cases, dead bodies are redeemed partial burnt/ completely. In such cases, identification of an individual from highly burnt remnants is established by DNA profile. It can’t be neglected that hard tissues (bones and teeth) can bear extreme fire impact and often attained with some partial flesh pieces. Although, a considerable degree of degradation may occur in DNA retrieved from burnt bone pieces. This study is mainly focused upon the extraction and quantification of DNA profile generated from such remnants. Effect of fire on DNA and extreme heat on blood, blood in form of prime source of DNA are believed to be no longer traceable after exposure to a temperature of 1000°C. The outcome of this research will help researchers to quantify DNA profile, identify the deceased person/ dead bodies from such cases.
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