Molecular detection of some virulence gene of Helicobacter pylori isolated from patients with gastroenteritis in Al-Diwaniyah Governorate/Iraq
Keywords:
gastroenteritis, Helicobcter pylori, molecular detection, virulence factorsAbstract
Infection with Helicobacter pylori is one of the most common chronic bacterial infections worldwide. So, This study aimed to detect Helicobacter pylori in patients with gastroenteritis symptoms by molecular method Polymeraee chain reaction (PCR) . This study was conducted in AL- Diwaniyah province/Iraq and included 40 patients with age ranged from 11 to 56 years and they were males and females. The Physician diagnosed patients with stomach and intestinal infections. Samples were collected from the Consultant Internal Medicine and the Division of Gastroenterology at Al-Diwaniyah Teaching Hospital during the period from October 2021 to March 2022 . Tissue biopsy samples were collected from patients suffering from gastroenteritis in the Gastrointestinal Division. Three tissue biopsies were removed from the gastric antrum of each patient using forceps for endoscopy. The total number of samples was 20. Also, 20 Stool samples were collected from patients and then placed in a sterile container. Helicobacter pylori were diagnosed according to the diagnostic gene 16SrRNA in 12 samples with a percentage of 30% .
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