Molecular genotyping of enterococcus faecalis isolated from clinical sources by BOX-PCR and ERIC-PCR

Bashir H. Hassoun; Ghaidaa J. Mohammed

doi:10.53730/ijhs.v6nS1.7857

Authors

Bashir H. Hassoun University of Al-Qadisiyah/College of Science /Department of Biology
Ghaidaa J. Mohammed University of Al-Qadisiyah/College of Science /Department of Biology

Keywords:

Clinical specimens, Enterococcus faecalis, Antibiotic resistance, ERIC PCR, BOX PCR

Abstract

Gram-positive bacteria, Enterococcus faecalis, cause serious nosocomial infections such as UTI, bloodstream infections, and endocarditis. The study's aimed to isolate and identify Enterococcus faecalis from various clinical specimens in Al-Diwanyiah province hospitals ,testing the sensitivity of isolates to antibiotics as well as to genotyping of E.faecalis isolates by BOX –PCR or ERIC-PCR Analysis. The study extended from October 2021 to January 2022. Biochemical tests, Vitek 2 and 16S rRNA analysis identified 37 bacteria as Enterococcus faecalis. Five clusters (I-V) and about 13 polymorphic variants showed among 20 isolates by ERIC PCR. Whereas, four cluster (I-IV) and 14 polymorphic variants were showed by BOX PCR. The results showed that that there is a slight difference between ERIC and BOX-PCR analysis for Enterococcus faecalis isolated from clinical samples.

Downloads

Download data is not yet available.

References

Ahmadi, F., Siasi, E., & Amini, K. (2019). Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR. Medical Journal of Tabriz University of Medical Sciences, 41(5), 16-24.

Al-Khamasi, H. S. K. (2014). Detection of some virulence factors of Vancomycin-Resistant Enterococci College of Science-University of Baghdad].

ALNAKSHABANDI, W. M., MERZA, N. S., KHALED, H. M., & JUBRAEL, J. M. (2020). STUDY GENETIC RELATIONSHIP AMONG ENTEROCOCCUS FAECALIS STRAINS COLLECTED FROM URINE HARBORED DIFFERENT VIRULENCE PROFILES USING ERIC-PCR ASSAY. Journal of Duhok University, 23(1), 63-68.

AL-saadi, S. A. A. (2013). Molecular Characterization of Some Virulence Factors of Clinical Isolates of Enterococcus faecalis Babylon University College of Medicine].

AL-Yassary, K. A. H. (2011). study of Antibiotic susceptibility and virulence determinants among Enterococcus faecalis isolated from patients with significant Bacteriuria in najaf collage of science , University of kufa ].

Bhardwaj, S., Dhawale, K. B. J., Patil, M., & Divase, S. (2013). Enterococcus faecium and Enterococcus faecalis, the nosocomial pathogens with special reference to multi-drug resistance and phenotypic characterization. International Journal of Pharmaceutical Science and Practice, 2(1), 1-10.

Bachtiar, E. W., Bachtiar, B. M., Dewiyani, S., & Surono Akbar, S. M. (2015). Enterococcus faecalis with capsule polysaccharides type 2 and biofilm‐forming capacity in Indonesians requiring endodontic treatment. Journal of investigative and clinical dentistry, 6(3), 197-205.

Brooks, G. F., Morse, S. A., Brooks, G. F., & Butel, J. S. (2004). Jawetz, Melnick, & Adelberg's Medical Microbiology. Lange Medical Books/McGraw-Hill, Medical Pub. Division.

Conceição, N., de Oliveira, C. d. C. H. B., da Silva, L. E. P., de Souza, L. R. C., & de Oliveira, A. G. (2012). Ampicillin susceptibility can predict in vitro susceptibility of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates to amoxicillin but not to imipenem and piperacillin. Journal of clinical microbiology, 50(11), 3729-3731.

CLSI, Clinical and Laboratory Standards Institute. (2020). Performance standards for antimicrobial susceptibility testing. Approved standard M100-S17.‏

Clewell, D. B., Weaver, K. E., Dunny, G. M., Coque, T. M., Francia, M. V., & Hayes, F. (2014). Extrachromosomal and mobile elements in enterococci: transmission, maintenance, and epidemiology.

hassan, A. y. (2012). isolation and diagnosis of Enterococcus faecalis from different clinical sources and study of the pathogenicity in experimental mice College of Education University of Tikrit

Hugonnet, J.-E., Mengin-Lecreulx, D., Monton, A., den Blaauwen, T., Carbonnelle, E., Veckerle, C., Yves, V. B., van Nieuwenhze, M., Bouchier, C., & Tu, K. (2016). Factors essential for L, D-transpeptidase-mediated peptidoglycan cross-linking and β-lactam resistance in Escherichia coli. Elife, 5, e19469.

Hussain, S. S. A. (2020). Detection of Virulence Genes of Enterococcus Faecalis Isolates In Hilla Ctiy University of Babylon College of Medicine].

Kristich, C. J., Rice, L. B., & Arias, C. A. (2014). Enterococcal infection—treatment and antibiotic resistance. Enterococci: from commensals to leading causes of drug resistant infection [Internet].

Kadim, I. T., Mahgoub, O., Baqir, S., Faye, B., & Purchas, R. (2015). Cultured meat from muscle stem cells: A review of challenges and prospects. Journal of Integrative Agriculture, 14(2), 222-233.

Khalid, H. M. (2016). Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq. Science Journal of University of Zakho, 4(1), 63-72.

Kristich, C. J., Rice, L. B., & Arias, C. A. (2014). Enterococcal infection—treatment and antibiotic resistance. Enterococci: from commensals to leading causes of drug resistant infection [Internet].

Lal, D., Verma, M., & Lal, R. (2011). Exploring internal features of 16S rRNA gene for identification of clinically relevant species of the genus Streptococcus. Annals of Clinical Microbiology and Antimicrobials, 10(1), 1-11.

Lee, G. (2013). Ciprofloxacin resistance in Enterococcus faecalis strains isolated from male patients with complicated urinary tract infection. Korean journal of urology, 54(6), 388-393.

Martín-Platero, A. M., Valdivia, E., Maqueda, M., & Martínez-Bueno, M. (2009). Characterization and safety evaluation of enterococci isolated from Spanish goats' milk cheeses. International journal of food microbiology, 132(1), 24-32.

Nayak, B. S., Badgley, B., & Harwood, V. J. (2011). Comparison of genotypic and phylogenetic relationships of environmental Enterococcus isolates by BOX-PCR typing and 16S rRNA gene sequencing. Applied and Environmental Microbiology, 77(14), 5050-5055.

Patel, J. B. (2001). 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Molecular diagnosis, 6(4), 313-321.

Pourakbari, B., Mahmoudi, S., Aghdam, M. K., Sabouni, F., Eshaghi, H., Alizadeh, S., & Mamishi, S. (2013). Clonal spread of vancomycin resistance Enterococcus faecalis in an Iranian referral pediatrics center. Journal of preventive medicine and hygiene, 54(2), 87.

Rams, T. E., Feik, D., Mortensen, J. E., Degener, J. E., & van Winkelhoff, A. J. (2013). Antibiotic susceptibility of periodontal Enterococcus faecalis. Journal of periodontology, 84(7), 1026-1033.

Syrmis, M. W., O'Carroll, M. R., Sloots, T. P., Coulter, C., Wainwright, C. E., Bell, S. C., & Nissen, M. D. (2004). Rapid genotyping of Pseudomonas aeruginosa isolates harboured by adult and paediatric patients with cystic fibrosis using repetitive-element-based PCR assays. Journal of medical microbiology, 53(11), 1089-1096.

Saadi, f. S. A. A. (2007). study the Resistance of Enterococcus faecalis that caused Urinary Tract Infection to some Antibiotic and its production for lactamase Enzymes mustansiriyah ].

Seo, Y., & Lee, G. (2013). Antimicrobial resistance pattern in Enterococcus faecalis strains isolated from expressed prostatic secretions of patients with chronic bacterial prostatitis. Korean journal of urology, 54(7), 477-481.

Wei, L., Wu, Q., Zhang, J., Guo, W., Chen, M., Xue, L., Wang, J., & Ma, L. (2017). Prevalence and genetic diversity of Enterococcus faecalis isolates from mineral water and spring water in China. Frontiers in microbiology, 8, 1109.

Weng, P. L., Ramli, R., Shamsudin, M. N., Cheah, Y.-K., & Hamat, R. A. (2013). High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multilocus sequence typing from a hospital in Malaysia. BioMed research international, 2013.

Werner, G., Fleige, C., Feßler, A. T., Timke, M., Kostrzewa, M., Zischka, M., Peters, T., Kaspar, H., & Schwarz, S. (2012). Improved identification including MALDI-TOF mass spectrometry analysis of group D streptococci from bovine mastitis and subsequent molecular characterization of corresponding Enterococcus faecalis and Enterococcus faecium isolates. Veterinary microbiology, 160(1-2), 162-169.

Weterings, V., Van Oosten, A., Nieuwkoop, E., Nelson, J., Voss, A., Wintermans, B., Van Lieshout, J., Kluytmans, J., & Veenemans, J. (2021). Management of a hospital-wide vancomycin-resistant Enterococcus faecium outbreak in a Dutch general hospital, 2014–2017: successful control using a restrictive screening strategy. Antimicrobial Resistance & Infection Control, 10(1), 1-10.

Zalipour, M., Esfahani, B. N., & Havaei, S. A. (2019). Phenotypic and genotypic characterization of glycopeptide, aminoglycoside and macrolide resistance among clinical isolates of Enterococcus faecalis: a multicenter based study. BMC Research Notes, 12(1), 1-8.