Comparison of melatonin levels in saliva in individuals with healthy periodontium, generalized chronic gingivitis and generalized chronic periodontitis

Trupti Rathi; Pratha Akolu; Amit Chaudhari; Vidya Dodwad; Nishita Bhosale; Tanvi Khot

doi:10.53730/ijhs.v6nS1.8570

Authors

Trupti Rathi
truptirathi1994@gmail.com
Post Graduate Student, Department of Periodontology, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India
Pratha Akolu Post Graduate Student, Department of Periodontology, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India
Amit Chaudhari Assistant Professor, Department of Periodontology, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India
Vidya Dodwad Head of Department, Department of Periodontology, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India
Nishita Bhosale Assistant Professor, Department of Periodontology, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India
Tanvi Khot Post Graduate Student, Department of Periodontology, Bharati Vidyapeeth Dental College and Hospital, Pune, Maharashtra, India

Keywords:

melatonin, ELISA, Hormone, gingivitis, periodontitis, healthy periodontium

Abstract

Introduction: Melatonin, is a hormone secreted by the pineal gland which activates several elements of the immune system that reduce tissue destruction during the inflammatory response, either directly by scavenging free radical, reactive oxygen species and reactive nitrogen species or indirectly by modulating the action of agents such as cytokines and adhesion molecules, which contribute to the advance of cell damage. Aim: The aim of the present study was to compare and evaluate melatonin levels in saliva in individuals with healthy periodontium, generalized chronic gingivitis and generalized chronic periodontitis. Materials and method: A total of 60 patients were examined and assessed and divided into 3 groups; Group 1: 20 individuals with healthy periodontium, Group 2: 20 individuals with generalised chronic gingivitis and Group 3: 20 individuals with generalised chronic periodontitis. Saliva samples were collected from each sample and melatonin levels were assessed using ELISA, a competitive immunoassay using a capture antibody technique. Results: Melatonin levels in saliva was highest in individuals with healthy periodontium when compared to gingivitis and periodontitis group which was highly significant and inversely proportional correlation was observed between periodontal index and melatonin levels which was statically significant.

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