Molecular investigation of cutaneous leishmaniasis in humans in Al-Najaf Al-Ashraf governorate
Keywords:
molecular investigation, cutaneous leishmaniasis, humansAbstract
The method of molecular diagnosis using PCR technology, phylogenetic tree and DNA sequencing was used for the purpose of microscopic examination and accurate characterization of L. Major parasite. Depending on the diagnostic gene (ITS1) with a size of 350 pb and 500 pb base pairs, the genetic tree was also analyzed and the similarity ratios were compared among the globally registered isolates using the program MEGA In order to compare the percentages of similarity between strains in this study with local and global isolates registered in the NCBI Global Gene Bank within the Data base , Mega 10 program was used . Global Isolates.The results of the molecular diagnosis PCR for the highest samples of the neighborhoods of the southern governorate showed (26) injuries with a percentage of 40 %, and the lowest in the governorate center (5) injuries with a percentage of 7.7 %, while the district of Kufa and the northern neighborhoods recorded (21) and (13) injuries with rates of 32.3 %, 20 And straight.
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