Evaluation of in vitro antibacterial activity and phytochemical screening of nigella sativa leaves extracts against human pathogenic bacteria

Abdul Jabbar; Furqan Ali Shah; Anum Ashraf; Gul Hassan; Erum Rehman; Sudhair Abbas Bangash; Rana Muhammad Kamran Shabbir; Nabiha Naeem; Irfan Ullah

doi:10.53730/ijhs.v6nS9.13781

Authors

Abdul Jabbar Department of Medical Lab Technology, University of Haripur, Haripur
Furqan Ali Shah Department of Pharmacology, Pak International Medical College
Anum Ashraf Department of Pharmacology, Allama Iqbal Medical College Lahore
Gul Hassan Department of Pharmacology, Pak International Medical College
Erum Rehman Department of Pharmacology, Peshawar Medical College, Warsak Road Peshawar
Sudhair Abbas Bangash Faculty of Life Science, Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, Pakistan
Rana Muhammad Kamran Shabbir Department of Zoology, Division of Science and Technology, University of Education Lahore
Nabiha Naeem Department of Life Sciences, School of Science, University of Management and Technology (UMT), Lahore, Pakistan
Irfan Ullah
irfan_btn@hotmail.com
Department of Life Sciences, School of Science, University of Management and Technology (UMT), Lahore, Pakistan

Keywords:

Antibacterial activity, K. pneumonia, multi-drug resistant, N. sativa, P. aeruginosa

Abstract

Objective: The current study objective was to explore the antimicrobial activity and phytochemical screening (Alkaloids, Flavonoid, Saponins, Phenolic compounds, terpenoid) of leaves extract of Nigella sativa. Study Design: Experimental study Place and duration of study: The current study was performed in Department Medical Laboratory Technology (MLT) at the University of Haripur. Haripur, Khyber Pakhtunkhwa, Pakistan from March 2021 to February 2022. Methods: Antimicrobial susceptibility test was done on Muller Hinton agar (MHA) using the disk diffusion method. Screening of N. sativa leaves extracts against Gram-positive bacteria Methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative bacteria Pseudomonas aeruginosa, Salmonella species, Escherichia coli and Klebsiella pneumonia were carried out using standard methods. Results: The results showed that ethanol, chloroform, hexane extract of nigella sativa had the best antimicrobial activity against P. aeruginosa, Methicillin-resistant S. aureus, Salmonella species, E. coli and K. pneumonia. Leaves extract of N. sativa were found to contain terpenoid, flavonoid, alkaloids, phenolic compounds except for saponins. Nigella sativa leaves showed good antibacterial activity against clinical multi-drug resistant bacteria. Conclusions: The results of this study provide useful bioactive compounds that show strong antibacterial activity against different multi drug resistant (MDR) including gram negative and gram positive bacteria.

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