Next-generation sequencing in laboratory practice: Applications and challenges
Keywords:
Laboratory Automation, Next-Generation Sequencing, Public Health Laboratories, Workflow Optimization, COVID-19 DiagnosticsAbstract
Background _ The COVID-19 pandemic has underscored the critical role of next-generation sequencing (NGS) in diagnostic testing and surveillance-based screening. Public health laboratories need to incorporate these advanced molecular technologies to enhance their capabilities. However, challenges such as a shortage of skilled personnel, lack of sequencing expertise, assay standardization issues, and workload management within turnaround times are common. Aim of Work – This perspective paper aims to shed light on the benefits and challenges of using laboratory automation for sequencing purposes. It explores strategies for implementation, including instrument selection, validation approaches, staff training, and troubleshooting. Methods – In order to locate journal publications, the Scopus, Web of Science, and PubMed academic databases were searched. The following selection criteria were utilized: Articles must be written in English, focus on normative reasons rather than merely empirical research, include an abstract for software analysis, and describe NGS technology. The paper reviews current literature on laboratory automation in the context of NGS, examines case studies of successful implementation, and discusses potential barriers and solutions.
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